Journal: PLoS ONE
Article Title: Gene Expression Changes in the Olfactory Bulb of Mice Induced by Exposure to Diesel Exhaust Are Dependent on Animal Rearing Environment
doi: 10.1371/journal.pone.0070145
Figure Lengend Snippet: Within each group, a fold change (C-DE/C-C, EE-DE/EE-C, and EE-DE/C-C) was calculated and log 2 transformed for all 116 spots on the microarray that did not have any missing values. These values were then hierarchically clustered using Euclidean distance metric and complete linkage. The colored images are presented as described: the color scale ranges from saturated green for log 2 ratios –3.0 and below to saturated red for log 2 ratios 3.0 and above. Gene expression profiles were divided into 3 clusters (clusters A, B, and C), and quantitative RT-PCR analysis was performed for genes surrounded by the red line in each cluster. Genes surrounded by black dotted line were the same gene derived from different spots.
Article Snippet: Each of the pooled RNA samples was labeled with Cy3 and hybridized to a SurePrint G3 Mouse GE 8×60K microarray (Agilent Technologies) consisting of 62,976 spots (28,620 genes) according to the protocol of DNA Chip Research Inc. (Kanagawa, Japan).
Techniques: Transformation Assay, Microarray, Expressing, Quantitative RT-PCR, Derivative Assay